Journal: STAR Protocols

Article Title: Volumetric super-resolution imaging by serial ultrasectioning and stochastic optical reconstruction microscopy in mouse neural tissue

doi: 10.1016/j.xpro.2021.100971

Figure Lengend Snippet:

Article Snippet: Cy-3B Mono NHS-ester , Cytiva , PA63101.

Techniques: Recombinant, Saline, Electron Microscopy, Plasmid Preparation, Software, Imaging, Control, Mass Measurement, Capsules, Microscopy, Staining, Adhesive, Spectrophotometry

Journal: Pharmaceutics

Article Title: Design and Evaluation of Autophagy-Inducing Particles for the Treatment of Abnormal Lipid Accumulation

doi: 10.3390/pharmaceutics14071379

Figure Lengend Snippet: In vivo and organ DiR fluorescence imaging of genetically obese ob/ob mice following a single IV injection (120 µL) of soluble fluorophore or fluorescent particles (0.25% w/v ). ( a ) Autophagy-inducing fluorescent particles (NP DiR T-B) and plain fluorescent particles (NP DiR) accumulate in the liver over the course of 72 h. Images are representative of scans from 3–4 mice per group. ( b ) Quantification of whole-body fluorescence up to 72 h post a single IV injection. The percentage values reported are calculated from the 5 min timepoint, when the maximal fluorescence was observed, and are reported as mean ± s.e.m. Results were analyzed using one-way ANOVA followed by Sidak’s multiple comparison test, which revealed no significant difference between the NP DiR and the NP DiR T-B group for all timepoints. ( c , d ) Organ biodistribution of fluorescent NPs 24 h after a single intravenous (IV) injection in ob/ob mice. n = 3–4 mice per group. Ex vivo DiR fluorescence imaging on main organs (liver, spleen, pancreas and kidneys) ( c ) and quantification of DiR fluorescence ( d ). The percentage values reported are calculated from total organ fluorescence and presented as mean ± s.e.m ( d ).

Article Snippet: Mice were immediately injected in the retro-orbital vein with 120 µL of either free fluorophore (Xenolight DiR-Fluorescent Dye, PerkinElmer, Waltham, MA, USA, 125964), fluorescent nanoparticles (NP DiR, Near Infra-Red Fluorescent i-Particles ® , Adjuvatis, France) or fluorescent Tat-Beclin nanoparticles (NP DiR T-B) of 0.25% w/v solid content.

Techniques: In Vivo, Fluorescence, Imaging, IV Injection, Comparison, Ex Vivo

Journal: Nucleic Acids Research

Article Title: Enhanced exon skipping and prolonged dystrophin restoration achieved by TfR1-targeted delivery of antisense oligonucleotide using FORCE conjugation in mdx mice

doi: 10.1093/nar/gkac641

Figure Lengend Snippet: FORCE–M23D structure, Fab internalization kinetics and FORCE–M23D TfR1 binding affinity in C2C12 mouse myoblasts. ( A ) Schematic representation of the FORCE–M23D conjugate. ( B ) Binding to murine TfR1 with the FORCE–M23D conjugate or with a negative control human-TfR1 specific conjugate by ELISA. ( C ) Uptake of anti-murine TfR1-Cypher5a conjugate and anti-human TfR1-Cypher5e conjugate in C2C12 myoblasts. Data represent mean ± SD. *** P < 0.001. A.U., arbitrary units; ELISA, enzyme-linked immunosorbent assay.

Article Snippet: The pH-sensitive dye Cypher5E was purchased from Cytiva, Malborough, MA (Cy5E; Cat. No. PA15405).

Techniques: Binding Assay, Negative Control, Enzyme-linked Immunosorbent Assay